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Heating the solution of RNA prior to incubation with Vaccinia capping enzyme
Kamal Singh
" Journal of Biological Chemistry 281
or 10 min at 65 °C in AMA (ammonia/methylamine
In vitro transcription (IVT) of chemically modified mRNA to modulate immunogenicity and improve expression profiles
EPO mRNA (N1-Me-Pseudo U) Enzyme & Related Buffer for mRNA Heating the solution of RNAEPO mRNA is a messenger RNA molecule that encodes erythropoietin (EPO). Through optimization of co transcription with cap addition, it has high translation efficiency and stability, while reducing immunogenicity. It is widely used in mRNA drug research and development and the evaluation of delivery systems. Concentration: 1. 0 mg mL Buffer: 1 mM Sodium Citrate pH 6. 4 Modifications: N1 Methyl Pseudo U Endotoxin: < 1 EU mg Bioanalyzer of Final Product: